By Walter Maechtle
This publication is split into chapters protecting instrumentation, sedimentation pace runs, density gradient runs, program examples and destiny advancements. specifically, the distinct software bankruptcy demonstrates the flexibility and tool of AUC via many attention-grabbing and critical business examples. therefore the booklet concentrates on useful points instead of info of centrifugation concept.
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Extra resources for Analytical Ultracentrifugation of Polymers and Nanoparticles (Springer Laboratory)
Hence, only a relative fringe shift ΔJ(r) with respect to the meniscus is measurable. Thus, only a relative concentration Δc(r) can be determined, too. In Sect. 8)), it will be shown for the case of equilibrium runs how the mass conservation law can be utilized to calculate J(rm ) and c(rm ). Again, the detecting system of the older Model E by Beckman served as basis for the development of the new XL-A/I interference optics [18, 19]. In comparison, major changes have been made to the light source and the detecting unit.
In comparison, major changes have been made to the light source and the detecting unit. The constantly burning mercury lamp as light source was replaced by a pulsed laser diode. A CCD camera replaced the photographic plate as detecting unit. M. Laue in , page 63). These data are collected online. More recently, signiﬁcant improvements have been realized by changing the CCD camera system to the next camera generation, especially with increased resolution. 36 2 Analytical Ultracentrifugation, Instrumentation Fig.
However, this shifting can be corrected easily. Furthermore, if a sample shows too much turbidity, the fringes on the CCD camera monitor appear faintly, and it could be possible that the Fourier transform analysis fails. 3 Schlieren Optics The Schlieren optics setup (as described above in Sect. 2 and Fig. 3) is similar to the setup of the Rayleigh interferometer but has a phase plate (or knife-edge) in the focus of the condenser lens as an additional element. As there is no commercially available system anymore, users are obliged to construct their own Schlieren optics, or transfer the Schlieren optics of an older Model E onto the Optima XL platform .
Analytical Ultracentrifugation of Polymers and Nanoparticles (Springer Laboratory) by Walter Maechtle