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By Harry ed. Sobotka

ISBN-10: 0120103044

ISBN-13: 9780120103041

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46,400408( 1949). E l . Ells, V. , The Lundegardh flame method of spectrographic analysis. J. Opt. SOC. Am. 31, 534-542 (1941). E2. Ells, V. , and Marshall, C. , The determination of exchangeable bases by the Lundegirdh spectrographic method. Soil Sci. Sac. Am. Proc. 4, 131-135 ( 1939). G1. Gaydon, A. , and Wolfhard, H. ” Chapman & Hall, London, 1953. G2. Griggs, M. , and Elledge, B. , Mineral analysis of biologic materials; use of Lundegirdh spectrographic method. Ind. Eng. , Anal. Ed. 13, 99-101 (1941).

London, E. C. 2), and the contents are mixed by gently inverting the tube several times, It is then centrifuged, and the clear supernatant is used. Urine. Urine is diluted 10- or 20-fold with diluting fluid (2). Twentyfour-hour urine specimens should be collected in a Winchester bottle containing 10 ml concentrated HCl in order to prevent precipitation of magnesium and calcium. Feces. Specimens are homogenized, and a sample is dried at 105" for 16 hours. The ash is dissolved in NHCl (200 ml HCl/g dry feces).

P. 40-60"). Obvious tendon is removed in the case of muscle. The powder is placed in the oven at 105"for 12 hours. A known weight (approximately 20 mg) of the dry fatfree tissue powder is weighed into a platinum crucible and ashed at 400". The ash is dissolved in diluting fluid ( 3 ) (4 m1/20 mg tissue powder). The ash solution is suitable for the determination of sodium and potassium by flame photometry as well as for calcium and magnesium. s. The methods described are directly applicable even when only a small volume of plasma or urine is available.

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Advances in Clinical Chemistry, Vol. 4 by Harry ed. Sobotka

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